🤓 Based on our data, we think this question is relevant for Professor Minto's class at IUPUI.
We are asked to determine if it's possible to determine the concentration of a certain substance where its absorbance is known and given that a certain absorbance from another substance with a known concentration from the spectrometer is known. Both measurements were measured under the same wavelength.
From the Beer-Lambert Law,
Where A is absorbance, e is the molar absorbtivity, b is the path length of the sample - that is, the path length of the cuvette in which the sample is contained, and c is the concentration of the compound in solution.
If someone measured a certain absorbance from a solution with a known concentration from the spectrometer, and then measured another solution of the same species at the same wavelength in the same spectrometer, is it possible to calculate the concentration of the second substance (which is currently an unknown)?