Problem: You digest DNA from the frog Xenopus laevis with HindIII that produces sticky DNA ends. You can ligate your DNA to : (check all that apply)A. HindIII sticky DNA ends from Xenopus laevisB. EcoRI sticky DNA ends from Xenopus laevisC. blunt DNA ends from bacteriaD. HindIII sticky DNA ends from bacteria

🤓 Based on our data, we think this question is relevant for Professor Kuta's class at TOWSON.

FREE Expert Solution

Sticky ends are double-stranded DNA fragments with each strand different in length due to the uneven cut in a restriction site's palindromic sequence. The DNA strands undergo hydrolysis in these sites as catalyzed by restriction enzymes.

The function of these restriction enzymes are well known in cloning applications as this enzyme allows easy integration of a particular DNA sequence to the original DNA strand to introduce one or more additional traits. 

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Problem Details

You digest DNA from the frog Xenopus laevis with HindIII that produces sticky DNA ends. You can ligate your DNA to : (check all that apply)

A. HindIII sticky DNA ends from Xenopus laevis

B. EcoRI sticky DNA ends from Xenopus laevis

C. blunt DNA ends from bacteria

D. HindIII sticky DNA ends from bacteria

Frequently Asked Questions

What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the DNA Cloning concept. You can view video lessons to learn DNA Cloning. Or if you need more DNA Cloning practice, you can also practice DNA Cloning practice problems.

What professor is this problem relevant for?

Based on our data, we think this problem is relevant for Professor Kuta's class at TOWSON.