Problem: Trypsin digestion of the polypeptide Asp-Arg-Val-Tyr-Lys-His-Pro-Phe gives:A. Asp-Arg, Val-Tyr-Lys, His, Pro-PheB. Asp, Arg-Val-Tyr, Lys-His-Pro-PheC. Asp-Arg, Val-Tyr-Lys, His-Pro-PheD. Asp, Arg, Val-Tyr, Lys, His-Pro-Phe

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The primary structure (i.e. sequence) of a polypeptide chain can be determined with the help of certain peptidases. These are enzymes known to hydrolyze specific peptide bonds. One example of this enzyme is trypsin, which exists naturalls in our digestive tract for the digestion of proteins.

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Problem Details

Trypsin digestion of the polypeptide Asp-Arg-Val-Tyr-Lys-His-Pro-Phe gives:

A. Asp-Arg, Val-Tyr-Lys, His, Pro-Phe

B. Asp, Arg-Val-Tyr, Lys-His-Pro-Phe

C. Asp-Arg, Val-Tyr-Lys, His-Pro-Phe

D. Asp, Arg, Val-Tyr, Lys, His-Pro-Phe