Problem: Jason is setting up a reaction with the restriction endonuclease EcoRI. The concentration of the EcoRI enzyme solution is 20,000 units/mL. Jason mixes the following in a test tube:4 uL dsDNA, 14 uL buffer, 2 uL EcoRIWhat is the final concentration of EcoRI enzyme in Jason's reaction?A. 2,222 units/mLB. 2,857 units/mLC. 2,000 units/mLD. 200,000 units/mL

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This is a simple dilution problem for the EcoRI enzyme solution. 

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Problem Details

Jason is setting up a reaction with the restriction endonuclease EcoRI. The concentration of the EcoRI enzyme solution is 20,000 units/mL. Jason mixes the following in a test tube:

4 uL dsDNA, 14 uL buffer, 2 uL EcoRI

What is the final concentration of EcoRI enzyme in Jason's reaction?

A. 2,222 units/mL

B. 2,857 units/mL

C. 2,000 units/mL

D. 200,000 units/mL

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What scientific concept do you need to know in order to solve this problem?

Our tutors have indicated that to solve this problem you will need to apply the DNA Cloning concept. You can view video lessons to learn DNA Cloning. Or if you need more DNA Cloning practice, you can also practice DNA Cloning practice problems.